October 2014 - Present, Chemistry Research Laboratory, University of Oxford;
Supervisor: Prof. Harry L. Anderson, FRS
Synthesis, characterization and testing of novel voltage sensitive dyes. I spent around a year in organic synthesis, charaterization and purification of novel voltage sensitive dyes. Characterization techniques include proton NMR, carbon NMR, mass spec, UV-Vis spectroscopy (for calculation of molar absorption coefficient), fluorescence spectroscopy (for calculation of quantum yield), electrochemistry and semi-emperical based MOPAC calculations. Currently, I am working full-time in electrophysiology and multi-photon microscopy. I have set up the patch-clamp system in our lab along with the multi-photon microscope. I am testing the voltage sensitivity of the novel dyes in HEK 293T cells through two-photon fluorescence and second harmonic generation by applying membrane potential through whole cell patch-clamp technique, under the microscope.
D.Phil. second rotation project: Synthesis of dyes;
July 2014 - October 2014, Chemistry Research Laboratory, University of Oxford;
Supervisor: Prof. Harry L. Anderson,FRS, Dr. Paul Karila (Cellectricon)
Derivatives of an organic compound were synthesized.
D.Phil. first rotation project: Structural and Functional analysis of Bromo Adjacent Homology (BAH) domains;
April 2014 - July 2014, Structural Genomics Consortium, University of Oxford;
Supervisor: Dr. Panagis Filippakopoulos, Prof. Stefan Knapp
Bromo-adjacent homology (BAH) domains are small evolutionary conserved β-sheet interaction modules found in proteins involved in transcriptional regulation. Structural Information is limited and their specific target linear motifs of recognition are not available. This project aims to perform the structural and functional analysis of BAH domains.
M.Sc. project: Engineering injectable polymeric implants for neurological applications;
December 2012 - August 2013, Nanomedicine Lab, University College London (UCL);
Supervisor: Professor Kostas Kostarelos
This project aimed at the development of an injectable electro-responsive hybrid polymeric implant (containing polymeric nanoparticles) for the pulsatile, targeted and controlled delivery of a biologically active molecule: retinoic acid. Retinoic acid is a growth factor which triggers the differentiation of primary neural cells into dopaminergic neurons
Undergraduate project: Preparation of gold nanoparticles-alpha lactalbumin protein conjugates for breast cancer therapy;
July 2011 - May 2012, NIT Rourkela;
Gold nanoparticles were synthesized by using a leaf water extract as reducing agent. Their conjugate with alpha-lactalbumin protein were prepared, characterized and administered on cultured MDAMB-231 cancer cell lines. The conjugates of nanoparticles and alpha-lactalbumin protein killed more than 50% of MDAMB-231 cancer cells
Undergraduate summer project: Studying Hydrophobicity of Gold nanoparticles by partition in Aqueous Two Phase System (ATPS);
May 2011 - July 2011, IIT-Kanpur;
Aqueous Two Phase Systems (ATPS) of Polyethylene glycol (PEG) and Dextran were prepared. Gold nanoparticles were synthesized by Fren’s method and then characterized. The relative surface hydrophobicity of gold nanoparticles was studied by their partition in Aqueous Two Phase Systems (ATPS)
Undergraduate summer project: Development of novel approach for concentration analysis in PEG-Dextran Aqueous Two Phase System(ATPS);
May 2011 - July 2011, IIT-Kanpur;
Anthrone assay and Ammonium Ferrothiocyanate assay were carried out for calculating the phase concentrations of Polyethylene glycol (PEG) and Dextran in PEG-Dextran Aqueous Two Phase System (ATPS) which is a novel approach. The concentration data thus calculated were compared with the data obtained from conventional method; the data from both the methods were found to be similar
1. Push-pull pyropheophorbides for nonlinear optical imaging
A. Khadria, Y. de Coene, P. Gawel, C. Roche, K. Clays, H.L. Anderson
Org. Biomol. Chem.2017, 15, 947-956.