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Alternative synthesis research in asthma drug project, research chemist (6 months), Novartis Ltd., Basel, Switzerland. My work was superwised by Dr. Christian Mathes and Dr. Philipp Lustenberger. The goal of my research was to explore more efficient alternatives for established synthetic routes, which could be applied to kilogram production scale synthesis. The scope of the reserched reactions mainly comprised reductive aminations of nitrogen-containing heterocycles using different borohydride reagents.
Design and synthesis of protein inhibitors as a strating point for a perspective CNS drug, MChem. thesis project in industry, Hoffmann-La Roche Ltd., Basel, Switzerland, supervised by Dr Christian Lerner, Dr. Andrew Thomas and Dr. Paul Clarke. In 2011 I joined Dr. Christian Lerner's laboratory, which designed and synthesised protein inhibitors for a perspective CNS desease drug. This project involved organic synthesis using parallel synthesis, medicinal chemistry, X-ray crystallography and biology. Here not only did I significantly improve my synthetic skills, but also developed my theoretical knowledge in such areas as ligand-protein interactions and brain signalling pathways. I had an opportunity to improve my practical skills in crystallography by constructing several crystal structures of ligand-protein co-crystals for my project. Thanks to monthly organised meetings, where we had to report on development in our project, I was able to significantly improve my ability to give scientific presentations.
Crystallisation of Lin28 protein, Summer internship, York Structural Biology Laboratory (YSBL), The University of York, York, UK, supervised by Prof. Fred Antson. I was working in thos research group during summer 2009. The goal of the project I joined was determination of RNA-binding protein Lin28 structure. The goal was to crystallize this protein, so that its structure could be elucidated by X-ray crystallography, and optimize condition for its crystallization. I did all the steps of the research process starting from E.coli transformation with corresponding vector, protein extraction from bacteria, protein purification using a broad variety of techniques and research on the best crystallization conditions. I have got experience at different analytical and purification techniques like different kinds of chromatography, various kinds of electrophoresis. Moreover, I was using basic biochemical protocols like DNA and plasmid purification, PCR and etc. In addition, I really advanced my understanding of protein chemistry, gene expression mechanisms and biology associated with protein-RNA interactions.