Synthetic Circuit Design

This course will cover modelling and analysis of synthetic genetic circuits. Topics covered will include logic circuits, standardization and registry of standard biological parts, libraries and chassis and the engineering design test cycle. Tools including CAD tools, Eugene, Clotho, BioFAB, GEC Compilers, Snapgene will be covered as well as best implementation of designs. In the second week information will be provided on promoter engineering and promoter interference/inducible expression, translation and RNA secondary structure, RBS and codon usage, CRISPR/RNA silencing, RNA circuits/riboswitches and ultimately protein engineering, maturation and degradation. Wetlab practical sessions will be spread throughout the 2 weeks.